Effector Biology

Eighty Known Effectors Aligned to H. glycines Genome V1

Eighty effectors have been previously identified in H. glycines (1-2). The mRNA sequences of these effectors were aligned to the H. glycines genome V1 to identify their genomic sources.  Many of the functions of these effectors have been characterized, some of include: defense suppression, alterations of plant hormone signaling, epigenetic modification, metabolic manipulation, and cytoskeletal modifications (reviewed by 3-6). Read more about Eighty Known Effectors Aligned to H. glycines Genome V1

H. glycines Genes with Promoters Enriched for DOG-box Motifs

DOG-box DNA motifs were enriched in the promoters of dorsal gland expressed genes in Globodera rostochiensis (1). As a modulus to identify putative effectors, we searched for a similar motif in H. glycines.  A set of DOG-box containing genes from G. rostochiensis and glutathione synthetase genes from both H. glycines and G. rostochiensis were used to refine a H. glycines DOG box using MEME.  The promoters of the genes in H. Read more about H. glycines Genes with Promoters Enriched for DOG-box Motifs

Secreted Genes in the H. glycines V1 genome

Because secreted proteins play a vital role in H. glycines parasitism, iwe surveyed the H. glycines annotation for genes with a signal peptide for secretion that also lack a transmembrane domain (1).  A total of 2,222 genes were classified as secreted using Phobius (2) and SignalP (3) annotations obtained using interproscan(4).  However, due to a coding error, these annotations were incomplete.  With SignalP 4.0 (3) we have identified a larger set of secreted genes, 4,656.

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